ELISA detection of Kudoa septempunctata in raw Paralichthys olivaceus (olive flounder) using a chicken anti-Kudoa antiserum.
نویسندگان
چکیده
Kudoa septempunctata is the causative agent of a foodborne disease associated with the consumption of raw Paralichthys olivaceus (olive flounder). Chickens were used to establish specific antibodies against K. septempunctata spores. A specific antiserum, CS#3, raised against sonicated spores, also recognized intact spores. The CS#3 antiserum showed high titers for sonicated and intact K. septempunctata spores and was suitable for both ELISA and immunohistochemical staining. Using homogenated raw olive flounder meat, the ELISA system detected more than 5.0×10(5) spores in 1 g of tissue, which was consistent with the number determined by microscopic examination. The preparation of rapid detection kits for K. septempunctata spores in P. olivaceus muscle tissue using immunochromatography with CS#3 antiserum should be useful for preventing the foodborne disease in the field.
منابع مشابه
Inter-laboratory validation and applications of quantitative real-time PCR for the detection of Kudoa septempunctata in olive flounder (Paralichthys olivaceus).
Kudoa septempunctata, a myxosporean parasite, was recently identified as the causative agent of food poisoning resulting from the consumption of raw olive flounder (Paralichthys olivaceus). A single blind inter-laboratory study, involving 5 laboratories, was conducted to validate a quantitative real-time PCR assay for the detection of the parasite. We obtained relatively constant values for log...
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ورودعنوان ژورنال:
- Biocontrol science
دوره 18 4 شماره
صفحات -
تاریخ انتشار 2013